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2009, 2010, 2012, 2013, 2014, 2016 and 2017
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RESEARCH AREAS > Biodevices and Diagnostics

Biodevices and Diagnostics

Biodevices and Diagnostics, which involve nanotechnology and microfabricated systems for rapid disease diagnosis, high-throughput biomarker and drug screening, and affinity reagent selection.

View research projects, research publications and research staff in this research area.

Research Projects

1. An Automated Device for Sample Preparation and Multiplexing PCR Detection

Guo Lin Xu, Rensheng Deng, James Tseng Ming Hsieh, Muhammad Nadjad Abdul Rahim and Jackie Y. Ying

This project aims to develop an automated molecular diagnosis device to identify bacteria and viruses directly from complex samples like body fluids and food substances. The patient or food samples are lysed and purified before undergoing polymerase chain reaction (PCR) for amplification and multiplex detection. Multiple bacteria and/or viruses can be detected at the same time from a single sample. The device will be designed to detect common food-borne pathogens and drug-resistant bacteria including Carbapenem-resistant enterobacteriaceae (CRE) and Vancomycin-resistant enterococci (VRE). The entire process can be completed within one hour in a microfluidic chip.

2. Antibody Engineering for Rapid Diagnostic Tests

Thai Leong Yap, Huajing Wang, Shin Yee Hong, Ying Ping Chua and William Sun

Rapid tests for the detection of infectious diseases can provide diagnosis at the point-of-care setting, enabling speedy and proper patient management. Rapid diagnostics, such as the lateral flow strip, require high-quality antibodies. We use a variety of engineering methods to isolate antibodies specific for pathogen proteins. These antibodies can then be incorporated into suitable assay platforms for the development of diagnostic tests. Our efforts are focused on generating rapid tests for diagnosing infections prevalent in Southeast Asia, such as dengue fever and Zika.

3. Circulating Tumor Cell Isolation for Non-Invasive Cancer Diagnosis and Prognosis

Min Hu, Jamie Mong, Jun-Li Shi, Min-Han Tan and Jackie Y. Ying

Circulating tumor cells (CTCs) are cells that detach from a primary tumor and travel in the bloodstream to other locations, leading to the spread of tumor in the body. Catching and analyzing CTCs can further our understanding of cancer metastasis and enhance the treatment of cancer patients. However, CTCs are very rare compared to the large number of blood cells in the body, and there is a lack of universal cancer cell biomarkers to distinguish them from other circulating cells. Based on size and deformability selection, IBN’s microsieve devices enable the effective isolation of circulating tumor cells from patients’ blood samples in minutes without the use of costly reagents and complex instruments. The microsieve technology permits a simple workflow, with rapid separation and cell characterization on the same device. Intact and viable isolated CTCs can be easily eluted for molecular diagnosis, drug screening and assay development.

4. Development of Peptides with High Binding Affinity and High Specificity for Diagnostic and Therapeutic Applications

Joo-Eun Jee, Li Qian Gao, Yong Siang Ong and Su Seong Lee

Currently, antibodies are widely used for detecting protein biomarkers, but their high cost and chemical instability are major issues. Using synthetic peptides, IBN is developing high-affinity, highly specific capture agents for biomarkers of infectious diseases, such as dengue and Zika. The protein capture agents can be produced by screening bead-based D-peptide libraries in a high-throughput platform. Compared to antibodies, these synthetic peptides are highly stable and inexpensive, making them ideal as protein capture agents for in vitro diagnostic devices, immunohistochemistry and flow cytometry. These peptides can also be used for therapeutic purposes.

5. Genetic Alphabet Expansion using Unnatural Base Pair Systems

Michiko Kimoto, Itaru Okamoto, Kiyofumi Hamashima, Ken-ichiro Matsunaga and Ichiro Hirao

The genetic information flow of the central dogma relies on only four nucleobases, which in turn constrains the Darwinian evolution of nucleic acids and proteins as functional molecules. IBN is developing a genetic alphabet expansion technology of DNA by creating extra base pairs, called unnatural base pairs. The unnatural base pairs exhibit high fidelity and efficiency in replication and/or transcription as a third base pair. The unnatural base pair systems can be used in a wide variety of biotechnology applications, such as PCR detection and aptamer generation.

6. High-Affinity DNA Aptamer Generation

Michiko Kimoto, Itaru Okamoto, Kiyofumi Hamashima, Nur-Afidah Binte Mohamed Suhaimi, Ken-ichiro Matsunaga and Ichiro Hirao

IBN has successfully generated high-affinity DNA aptamers that bind to target proteins and cells by a new evolutionary engineering method called ExSELEX (genetic alphabet Expansion for Systematic Evolution of Ligands by EXponential enrichment) using our unnatural base pair technology. We have also created another technology, called mini-hairpin, to stabilize high-affinity DNA aptamers against heat and nucleases. We are developing diagnostic and therapeutic methods using these new technologies for cancer and infectious diseases.

7. Nucleic Acid Detection Platforms for Molecular Diagnostics and Pharmacogenomics

Yanbing Zu and Jackie Y. Ying

The genome is the database containing all cellular information in human cells, including when and how the cells will grow. Any alteration to our genes can cause functional disruptions, resulting in diseases and uncontrolled growth, i.e. cancer. These alterations may also cause people to become less sensitive or overly responsive to certain drugs, which may lead to side effects and even death. Using gold-morpholino nanoprobes, we have developed a highly sensitive and specific method to detect the presence or alteration of certain genes by a color change. The results can be interpreted easily and obtained inexpensively. Our method is also applicable for the detection of harmful bacteria. We are developing a number of clinically important assay kits for the healthcare industry.

8. Paper-Based Assays

Jun Hui Soh, Benjamin Tai, Thai Leong Yap, Aysha Farwin, Min Hu, Huajing Wang, Shin Yee Hong, Ying Ping Chua, Kyun Hyun Lee, Huaqiang Zeng, William Sun and Jackie Y. Ying

IBN is developing diagnostic platforms for infectious diseases that can be translated into point-of-care rapid test kits. The new paper-based assays can detect diseases such as dengue, Zika, hepatitis, and sexually transmitted diseases, using a small volume of blood or non-invasive saliva and urine samples. They are designed to be fast, accurate and convenient for self-testing so as to facilitate health monitoring. We are incorporating new reagents, such as aptamers, short peptides, and new antibodies to further improve the assay’s performance. Paper-based assays are also being developed for food safety and environment monitoring applications, as well as to monitor cellular metabolic activities.

9. Peptide Tags for Live Cell Imaging

Joo-Eun Jee and Su Seong Lee

A universal tagging method for fluorescence probes is important for imaging complex biological events through live cell imaging. The current tagging methods employing fluorescence proteins, such as green fluorescent protein, have some disadvantages, including interference of the bulky fluorescence tags in the physiological activities of the target proteins. Many research groups have been developing smaller probes to overcome this problem. In this project, we aim to develop small peptide tags that can bind strongly and selectively to a specific fluorescence dye. The identified peptide tags can be genetically incorporated into target proteins and bind specifically to the fluorescent dyes for imaging purpose. In particular, we are targeting intracellular imaging using fluorescence dyes with cell-penetrating ability.

10. Practical Assays Predicting Diagnosis, Drug Response and Prognosis in Oncology

Michelle Pek, Jamie Mong, Jun Li Shi, Rachel Sim, Siting Zhang and Min-Han Tan

We focus on developing practical assays that will be deployed in the clinic. We are interested in developing working assays for predicting outcomes in lung, breast, renal and colorectal cancers, as well as for adverse drug reactions in non-oncological settings. To support assay development, we work with patient-derived material and create customized animal models.

11. Sensor System for Diaper Wetness and Feces Monitoring

Rensheng Deng, Guo Lin Xu, Jun Hui Soh, Min Hu and Jackie Y. Ying

Effective incontinence management is important to improve the care of elderly patients, especially those with aphasia. We are developing a diaper wetness and feces monitoring sensor system. Our sensors are cost-effective and able to transmit output voltage signals that correspond to wetness level and amount of feces. This network-based monitoring system can be used in hospitals and nursing homes.

Research Publications

  1. K. H. Lee, K. Hamashima, M. Kimoto and I. Hirao, "Genetic Alphabet Expansion Biotechnology by Creating Unnatural Base Pairs," Current Opinion in Biotechnology, 51 (2018) 8-15

  2. M. Kimoto and I. Hirao, "Unique Thermal Stability of Unnatural Hydrophobic Ds Bases in Double-Stranded DNAs," ACS Synthetic Biology, (2017) DOI: 10.1021/acssynbio.7b00165

  3. J. H. Soh, Y. Lin, M. R. Thomas, N. Todorova, C. Kallepitis, I. Yarovsky, J. Y. Ying and M. M. Stevens, "Distinct Bimodal Roles of Aromatic Molecules in Controlling Gold Nanorod Growth for Biosensing," Advanced Functional Materials, (2017) DOI: 10.1002/adfm.201700523

  4. S. L. Kong, X. Liu, N-A. Mohamed Suhaimi, K. Koh, M. Hu, D. Lee, I. Cima, W. M. Phyo, E. Lee, J. A. Tai, Y. M. Foong, J. Vo, P. K. Koh, T. Zhang, J. Y. Ying, B. Lim, M. Han Tan and A. M. Hillmer, "Molecular Characterization of Circulating Colorectal Tumor Cells Defines Genetic Signatures for Individualized Cancer Care," Oncotarget, (2017) DOI: 10.18632/oncotarget.19138

  5. K. H. Lee and H. Q. Zeng, "A General Double Library SELEX Strategy for Aptamer Selection Using Unmodified Nonimmobilized Targets," Analytical and Bioanalytical Chemistry, (2017) DOI: 10.1007/s00216-017-0454-z

  6. A. Marx, K. Betz, M. Kimoto, K. Diederichs and I. Hirao, "Structural Basis for Expansion of the Genetic Alphabet by An Artificial Base Pair," Angewandte Chemie International Edition, (2017) DOI: 10.1002/anie.201704190

  7. N.-A. Mohamed Suhaimi, W. M. Phyo, H. Y. Yap, S. Heng, X. Wei, Y. Choudhury, W. J. Tan, L. A. Peng, R. Foo, S. Tan, Z. Tiang, C. F. Wong, P. K. Koh and M. H. Tan, "Metformin Inhibits Cellular Proliferation and Bioenergetics in Colorectal Cancer Patient-Derived Xenografts," Molecular Cancer Therapeutics, (2017) DOI: 10.1158/1535-7163.MCT-16-0793

  8. J. H. Vo, W. L. Nei, M. Hu, W. M. Phyo, F. Wang, K. W. Fong, T. Tan, Y. L. Soong, S. L. Cheah, K. Sommat, H. Low, B. Ling, J. Ng, W. L. Tan, K. S. Chan, L. Oon, J. Y. Ying and M.-H. Tan, "Comparison of Circulating Tumor Cells and Circulating Cell-Free Epstein-Barr Virus DNA in Patients with Nasopharyngeal Carcinoma Undergoing Radiotherapy," Scientific Reports, 6 (2016) 13

  9. K. Matsunaga, M. Kimoto and I. Hirao, "High-Affinity DNA Aptamer Generation Targeting von Willebrand Factor A1-Domain by Genetic Alphabet Expansion SELEX (ExSELEX) Using Two Types of Libraries Composed of Five Different Bases," Journal of the American Chemical Society, (2016) DOI: 10.1021/jacs.6b10767

  10. J. Shen, C. Ren and H. Zeng, "Tuning Cation-Binding Selectivity and Capacity via Side Chain-Dependent Molecular Packing in the Solid State," Chemical Communications, (2016) DOI: 10.1039/C6CC04526J

  11. M. Kimoto, M. Nakamura and I. Hirao, "Post-ExSELEX Stabilization of an Unnatural-Base DNA Aptamer Targeting VEGF165 Toward Pharmaceutical Applications," Nucleic Acids Research, (2016) DOI: 10.1093/nar/gkw619

  12. I. Cima, S. L. Kong, D. Sengupta, I. B. Tan, W. M. Phyo, D. Lee, M. Hu, C. Iliescu, I. Alexander, W. L. Goh, M. Rahmani, N. A. Mohd Suhaimi, J. H. Vo, J. A. Tai, J. H. Tan, C. Chua, R. Ten, W. J. Lim, M. H. Chew, C. A. E. Hauser, R. M. van Dam, W. Y. Lim, S. Prabhakar, B. Lim, P. K. Koh, P. Robson, J. Y. Ying, A. M. Hillmer and M. H. Tan, "Tumor-Derived Circulating Endothelial Cell Clusters in Colorectal Cancer," Science Translational Medicine, 8[345] (2016) 345ra89

  13. Y. Huo and H. Zeng, "“Sticky”-Ends-Guided Creation of Functional Hollow Nanopores for Guest Encapsulation and Water Transport," Accounts of Chemical Research, 49[5] (2016) 922-930

  14. W. J. Tan, I. Cima, Y. Choudhury, X. Wei, J. C. T. Lim, A. A. Thike, M. H. Tan and P. H. Tan, "A Five-Gene Reverse Transcription-PCR Assay for Pre-Operative Classification of Breast Fibroepithelial Lesions," Breast Cancer Research, (2016) DOI: 10.1186/s13058-016-0692-6

  15. N. Hong, M. Li, Y. Yuan, T. Wang, M. Yi, H. Xu, H. Zeng, J. Song, and Y. Hong, "Dnd Is a Critical Specifier of Primordial Germ Cells in the Medaka Fish," Stem Cell Reports, 6 (2016) 1-11

  16. L. Gao, S. S. Lee, J. Chen, H. Sun, Y. Zhao, Z. Chai, Y. Hu, "High-Throughput Screening of Substrate Specificity for Protein Tyrosine Phosphatases (PTPs) on Phosphopeptide Microarrays," Methods in Molecular Biology, 1368 (2015) 181-196

  17. Y. Zhang and J. Y. Ying, "Homogeneous Immunochemical Assay on the Lateral Flow Strip for Measurement of DNase I Activity," Analytical Chemistry, (2015) DOI: 10.1021/acs.analchem.5b02658

  18. Y. Zhang and J. Y. Ying, "Homogeneous Immunochemical Assay on the Lateral Flow Strip for Rapid Measurement of DNase I Activity," Analytical Chemistry, (2015) DOI: 10.1021/acs.analchem.5b02658

  19. Z. Zheng, X. Wei, A. Hildebrandt and B. Schmidt, "A Computational Method for Studying the Relation Between Alternative Splicing and DNA Methylation," Nucleic Acids Research, (2015) DOI: 10.1093/nar/gkv906

  20. S. Roy, J. H. Soh and J. Y. Ying, "A Microarray Platform for Detecting Disease-Specific Circulating miRNA in Human Serum," Biosensors and Bioelectronics, 75 (2015) 238-246

  21. Y. H. Chu, H. Li, H. S. Tan, J. Lai, W. M. Phyo, Y. Choudhury, R. Kanesvaran, N. M. Chau, C. K. Toh, Q. S. Ng, P. H. Tan, B. Chowbay and M.-H. Tan, "Association of ABCB1 and FLT3 Polymorphisms with Toxicities and Survival in Asian Patients Receiving Sunitinib for Renal Cell Carcinoma," PLoS ONE, 10 (2015) e0134102

  22. W. J. Tan, J. Yan, S. Xu, A. A. Thike, B. H. Bay, H. Yu, M.-H. Tan and P. H. Tan, "Second Harmonic Generation Microcopy is a Novel Technique for Differential Diagnosis of Breast Fibroepithelial Lesions," Journal of Clinical Pathology, (2015) DOI: 10.1136/jclinpath-2015-203231

  23. G. Marinaro, A. Accardo, N. Benseny-Cases, M. Burghammer, H. Castillo-Michel, M. Cotte, S. Dante, F. De Angelis, E. Di Cola, E. Di Fabrizio, C. Hauser and C. Riekel, "Probing Droplets with Biological Colloidal Suspensions on Smart Surfaces by Synchrotron Radiation Micro- and Nano-Beams," Optics and Lasers in Engineering, (2015) DOI: 10.1016/j.optlaseng.2015.03.004i

  24. W. Wang, L. Qi, M.-H. Tan, Z. Zhang, J. Du, X. Wei and X. Yao, "Effect of Platelet-Derived Growth Factor-B on Renal Cell Carcinoma Growth and Progression," Urologic Oncology, (2015) DOI: 10.1016/j.urolonc.2014.12.015

  25. Y. Zhang,J. Bai, H. Wu and J. Y.Ying, "Trapping Cells in Paper for White Blood Cell Count," Biosensors and Bioelectronics, 69 (2015) 121-127

  26. P. Neuzil, W. Sun, T. Karasek and A. Manz, "Nanoliter-Sized Overheated Reactor," Applied Physics Letters, 106 (2015) 024104

  27. Y. Zhang, J. Bai and J. Y. Ying, "A Stacking Flow Immunoassay for the Detection of Dengue-Specific Immunoglobins in Salivary Fluid," Lab on a Chip, 15 (2015) 1465-1471

  28. N. A. Mohd Suhaimi, Y. M. Foong, D. Y. S. Lee, W. M. Phyo, I. Cima, E. X. W. Lee, W. L. Goh, W. Y. Lim, K. S. Chia, S. L. Kong, M. Gong, B. Lim, A. M. Hillmer, P. K. Koh, J. Y. Ying and M. H. Tan, "Non-Invasive Sensitive Detection of KRAS and BRAF Mutation in Circulating Tumor Cells of Colorectal Cancer Patients," Molecular Oncology, (2015) DOI: 10.1016/j.molonc.2014.12.011

  29. Y. Zhang and T. Wang, "High-Resolution Quantification by Charge-Dominant Electrophoretic Mobility Shift of Quantum Dots," Electrophoresis, (2014) DOI: 10.1002/elps.201400577

  30. H. S. Tan, H. Li, Y. W. Hong, C. K. Toh, A. Wong, G. Lopes, M. H. Tay, A. Chan, X. Yao, T. Tang, Q. S. Ng, R. Kanesvaran, N. M. Chau and M. H. Tan, "Efficacy and Safety of an Attenuated-Dose Sunitinib Regimen in Metastatic Renal Cell Carcinoma: Results from a Prospective Registry in Singapore," Clinical Genitourinary Cancer, (2014) DOI: 10.1016/j.clgc.2014.11.004

  31. Y. Choudhury, X. Wei, Y. H. Chu, L. G. Ng, H. S. Ta, V. Koh, A. A. Thike, E. Poon, Q. S. Ng, C. K. Toh, R. Kanesvaran, P. H. Tan, M. H. Tan, "A Multigene Assay Identifying Distinct Prognostic Subtypes of Clear Cell Renal Cell Carcinoma with Differential Response to Tyrosine Kinase Inhibition," European Urology, 67[1] (2014) 17-20

  32. Y. Zu, M. H. Tan, B. Chowbay, S. C. Lee, H. Yap, M. T. M. Lee, L-S. Lu, C-P. Chang and J. Y. Ying, "Nanoprobe-based Genetic Testing," Nano Today, 9[2] (2014) 166-171

  33. C. A. E. Hauser, S. Maurer-Strohb and I. C. Martins, "Amyloid-Based Nanosensors and Nanodevices," Chemical Society Reviews, 43 (2014) 5326-5345

  34. D. P. Poenar, C. Iliescu, J. Boulaire and H. Yu, "Label-Free Virus Identification and Characterization Using Electrochemical Impedance Spectroscopy," Electrophoresis, (2014) DOI: 10.1002/elps.201300368

  35. F. C. Tay, W. K. Tan, S.-L. Goh, C. J. A. Ramachandra, C.-H. Lau, H. Zhu, C. Chen, S. Du, R. Z. Phang, M. Shahbazi, W. Fan and S. Wang, "Targeted Transgene Insertion into the AAVS1 Locus Driven by Baculoviral Vector-Mediated Zinc Finger Nuclease Expression in Human Induced Pluripotent Stem Cells," The Journal of Gene Medicine, 15[10] (2013) 384-395

  36. T. T. Y. Tan, M. R. Reithofer, E. Y. Chen, A. G. Menon, T. S. A. Hor, J. Xu, and J. M. Chin, "Tuning Omniphobicity via Morphological Control of Metal-Organic Framework Functionalized Surfaces," Journal of the American Chemical Society,, 135[44] (2013) 16272-16275

  37. Y. Zhang, D. J. Shin and T.-H. Wang, "Serial Dilution via Surface Energy Trap-Assisted Magnetic Droplet Manipulation," Lab on a Chip, 13 (2013) 4827-4831

  38. D. Y. C. Heng, T. K. Choueiri, B. I. Rini, J. Lee, T. Yuasa, S. K. Pal, S. Srinivas, G. A. Bjarnason, J. J. Knox, M. MacKenzie, U. N. Vaishampayan, M. H. Tan, S. Y. Rha, F. Donskov, N. Agarwal, C. Kollmannsberger, S. North and L. A. Wood., "Outcomes of Patients with Metastatic Renal Cell Carcinoma That Do Not Meet Eligibility Criteria for Clinical Trials," Annals of Oncology, 25 (2013) 149-154

  39. B. Keeley, A. Stark, T. R. Pisanic II, R. Kwak, Y. Zhang, J. Wrangle, S. B. Baylin, J. G. Herman, N. Ahuja, M. V. Brock and T. H. Wang, "Extraction and Processing of Circulating DNA from Large Sample Volumes Using Methylation on Beads for The Detection of Rare Epigenetic Events," Clinica Chimica Acta, 425 (2013) 169-175

  40. N. Kroeger, T. K. Choueiri, J. L. Lee, G. A. Bjarnason, J. J. Knox, M. J. Mackenzie, L. Wood, S. Srinivas, U. N. Vaishamayan, S. Y. Rha, S. K. Pal, T. Yuasa, F. Donskov, N. Agarwal, M. H. Tan, A. Bamias, C. K. Kollmannsberger, S. A. North, B. I. Rini and D. Y. Heng, "Survival Outcome and Treatment Response of Patients with Late Relapse from Renal Cell Carcinoma in the Era of Targeted Therapy," European Urology, (2013) DOI: 10.1016/j.eururo.2013.07.031

  41. A. Mishra, K.-H. Chan, M. R. Reithofer and C. A. E. Hauser, "Influence of Metal Salts on the Hydrogelation Properties of Ultrashort Aliphatic Peptides," RSC Advances, 3 (2013) 9985-9993

  42. N. Kroeger, W. Xie, J. L. Lee, G. A. Bjarnason, J. J. Knox, M. J. Mackenzie, L. Wood, S. Srinivas, U. N. Vaishamayan, S. Y. Rha, S. K. Pal, T. Yuasa, F. Donskov, N. Agarwal, C. K. Kollmannsberger, M. H. Tan, S. A. North, B. I. Rini, T. K. Choueiri and D. Y. Heng, "Metastatic Non-Clear Cell Renal Cell Carcinoma Treated with Targeted Therapy Agents: Characterization of Survival Outcome and Application of the International mRCC Database Consortium Criteria," Cancer, 119 (2013) 2999-3006

  43. W. Y. Seow and C. A. E. Hauser, "A Convenient and Minimally-Invasive Method to Study Gel Formation by Surface Tension Measurements," Journal of Chemical Science and Technology, 2[1] (2013) 5-9

  44. L. C. Harshman, W. Xie, G. A. Bjarnason, J. J. Knox, M. MacKenzie, L. Wood, S. Srinivas, U. N. Vaishampayan, M. H. Tan, S. Y. Rha, F. Donskov, N. Agarwal, C. Kollmannsberger, S. North, B. I. Rini, D. Y. C. Heng and Toni K. Choueiri, "Conditional Survival of Patients with Metastatic Renal-Cell Carcinoma Treated with VEGF-Targeted Therapy: A Population-Based Study," Lancet Oncology, 13[9] (2012) 927-935

  45. X. Chen, A. Ying, Z. Gao, "Highly Sensitive and Selective Colorimetric Genotyping of Single-Nucleotide Polymorphisms Based on Enzyme-Amplified Ligation on Magnetic Beads," Biosensors and Bioelectronics, 36[1] (2012) 89-94

  46. Y. Y. Lee, K. Narayanan, S. J. Gao, J. Y. Ying, "Elucidating Drug Resistance Properties in Scarce Cancer Stem Cells using Droplet Microarray," Nano Today, 7[1] (2012) 29-34

  47. W. Y. Chay, W. S. Ong, P. H. Tan, N. Q. J. Leo, G. H. Ho, C. S. Wong, K. S. Chia, K. Y. Chow, M.-H. Tan, and P. Ang, "Validation of the Gail Model for Predicting Individual Breast Cancer Risk in a Prospective Nationwide Study of 28,104 Singapore Women," Breast Cancer Research, 14[1] (2012) R19

  48. L. S. Lee, and M.-H. Tan, "Predictive Models for the Practical Management of Renal Cell Carcinoma," Nature Reviews Urology, 9[2] (2012) 73-84

  49. D. Y. Heng, M. MacKenzie, U. Vaishampayan, J. J. Knox, G. A. Bjarnason, M. H. Tan, L. Wood, F. Donskov, C. Kollmannsberger, S. North, F. Donskov, B. I. Rini and T. K. Choueiri, "Primary Anti-Vascular Endothelial Growth Factor (VEGF)-Refractory Metastatic Renal Cell Carcinoma: Clinical Characteristics, Risk Factors and Subsequent Therapy," The Annals of Oncology, (2011) DOI: 10.1093/annonc/mdr533

  50. P. H. Tan, A. A. Thike, W. J. Tan, M. M. Thu, I. Busmanis, H. Li, W. Y. Chay and M. H. Tan, "Predicting Clinical Behavior of Breast Phyllodes Tumors: A Nomogram Based on Histological Criteria and Surgical Margins," Journal of Clinical Pathology, 65[1] (2011) 69-76

  51. G. Xu, D. Y. S. Lee, H. Xie, D. Chiew, T.-M. Hsieh, E. Mohamed Ali, X. L. Looi, M.-H. Li and J. Y. Ying, "A Self-Contained Polymeric Cartridge for Automated Biological Sample Preparation," Biomicrofluidics, 5 (2011) 34107

  52. J. Zhang, M. C. Pearce, B. P. Ting and J. Y. Ying, "Ultrasensitive Electrochemical Immunosensor Employing Glucose Oxidase Catalyzed Deposition of Gold Nanoparticles for Signal Amplification," Biosensors and Bioelectronics, 27[1] (2011) 53-57

  53. G. Yi, Y. F. Peng and Z. Gao, "Strong Red-Emitting Near-Infrared-to-Visible Upconversion Fluorescent Nanoparticles," Chemistry of Materials, 23 (2011) 2729-2734

  54. Y. Zu, A. L. Ting, G. Yi and Z. Gao, "Sequence-Selective Recognition of Nucleic Acids Under Extremely Low Salt Conditions Using Nanoparticle Probes," Analytical Chemistry, 83 (2011) 4090-4094

  55. S. Roy, J. H. Soh and Z. Gao, "A Microfluidic-Assisted Microarray for Ultrasensitive Detection of miRNA Under an Optical Microscope," Lab on a Chip, 11 (2011) 1886-1894

  56. H. Taylor, D. Boning and C. Iliescu, "A Razor-Blade Test of the Demolding Energy in a Thermoplastic Embossing Process," Journal of Micromechanics and Microengineering, 21[6] (2011) 67002

  57. X. Chen, Y. Zu, H. Xie, A. Muhammad Kemas and Z. Gao, "Coordination of Mercury(II) to Gold Nanoparticle Associated Nitrotriazole Towards Sensitive Colorimetric Detection of Mercuric Ion with Tunable Dynamic Range," Analyst, 136[8] (2011) 1690-1696

  58. C. Iliescu, M. Avram, B. Chen, A. Popescu, V. Dumitrescu, D. P. Poenar, A. Sterian, D. Vrtacnik, S. Amon and P. Sterian, "Residual Stress in Thin Films PECVD Depositions: A Review," Journal of Optoelectronics and Advanced Materials, 13 (2011) 387-394

  59. Z. Gao and Y. F. Peng, "A Highly Sensitive and Specific Biosensor for Ligation- and PCR-Free Detection of MicroRNAs," Biosensors and Bioelectronics, 26[9] (2011) 3768-3773

  60. Y. F. Peng, X. J. Chen, G. S. Yi and Z. Q. Gao, "Mechanism of the Oxidation of Organic Dyes in the Presence of Nanoceria," Chemical Communications, 47 (2011) 2916-2918

  61. Y. F. Peng and Z. Q. Gao, "Amplified Detection of MicroRNA Based on RuO2 Nanoparticle-Initiated Deposition of an Insulating Film," Analytical Chemistry, 83[3] (2011) 820-827

  62. Y. Zu, A. L. Ting and Z. Gao, "Visualizing Low-Level Point Mutations: Enzyme-Like Selectivity Offered by Nanoparticle Probes," Small, 7[3] (2010) 306-310

  63. G. L. Xu, F. E. H. Tay, G. Tresset, F. S. Iliescu, A. Avram and C. Iliescu, "Recent Trends in Dielectrophoresis," Informacije Midem - Journal of Microelectronics Electronic Components and Materials, 40 (2010) 253-262

  64. K.-J. Leck, S. Zhang and C. Hauser, "Study of Bioengineered Zebra Fish Olfactory Receptor 131-2: Receptor Purification and Secondary Structure Analysis," PLoS ONE, 5[11] (2010) 2780-2790

  65. N. C. Tansil, E. A. B. Kantchev, Z. Gao and H.-h. Yu, "Electropolymerization of Intercalator-Grafted Conducting Polymer for Direct and Amplified DNA Detection," Chemical Communications, 47[5] (2010) 1533-1535

  66. Y. Peng, G. Yi and Z. Gao, "A Highly Sensitive MicroRNA Biosensor Based on Ruthenium Oxide Nanoparticle-Initiated Polymerization of Aniline," Chemical Communications, 46[48] (2010) 9131-9133

  67. G. Xu, T.-M. Hsieh, D. Y. S. Lee, E. Mohamed Ali, H. Xie, X. L. Looi, E. S.-C. Koay, M.-H. Li and J. Y. Ying, "A Self-Contained All-in-One Cartridge for Sample Preparation and Real-Time PCR in Rapid Influenza Diagnosis," Lab on a Chip, 10[22] (2010) 3103-3111

  68. Y. Peng, X. Chen and Z. Gao, "Determination of Trace Amounts of Mercury Using Hierarchically Nanostructured Europium Oxide," Talanta, 82[5] (2010) 1924-1928

  69. J. Zhang, B. P. Ting, M. Khan, M. C. Pearce, Y. Yang, Z. Gao and J. Y. Ying, "Pt Nanoparticle Label-Mediated Deposition of Pt Catalyst for Ultrasensitive Electrochemical Immunosensors," Biosensors and Bioelectronics, 26[2] (2010) 418-423

  70. P. Neuzil, L. Novak, J. Pipper, S. Lee, L. F.-P. Ng and C. Zhang, "Rapid Detection of Viral RNA by a Pocket-Size Real-Time PCR System," Lab on a Chip, 10[19] (2010) 2632-2634

  71. W. C. Cheong, L. S. Lim, M. C. Huang, M. Bode and M.-H. Li, "New Insights into the De Novo Gene Synthesis Using the Automatic Kinetic Switch Approach," Analytical Biochemistry, 406[1] (2010) 51-60

  72. X. Chen, S. Roy, Y. Peng and Z. Gao, "Electrical Sensor Array for Polymerase Chain Reaction-Free Messenger RNA Expression Profiling," Analytical Chemistry, 82[14] (2010) 5958-5964

  73. C. Iliescu, G. Tresset, L. Yu and G. Xu, "3D Dielectrophoretic Chips: Trapping and Separation of Cell Populations," Romanian Journal of Information Science and Technology, 13[1] (2010) 49-64

  74. S. Roy and Z. Gao, "Direct-Write Fabrication of a Nanoscale Digital Logic Element on a Single Nanowire," Nanotechnology, 21[24] (2010) 245306

  75. S.-C. Luo, S.S. Liour and H.-H. Yu, "Perfluoro-Functionalized PEDOT Films with Controlled Morphology as Superhydrophobic Coatings and Biointerfaces with Enhanced Cell Adhesion," Chemical Communications, 46 (2010) 4731-4733

  76. C. Iliescu, G. Tresset, F. S. Iliescu and P. E. Sterian, "Live/Dead Cell Assay Based on Dielectrophoresis on a Chip," UPB Scientific Bulletin Series A - Applied Mathematics and Physics, 72[1] (2010) 33-44

  77. D. Lee, P. Sukumar, A. Mahyuddin, M. Choolani and G. Xu, "Separation of Model Mixtures of Epsilon-Globin Positive Fetal Nucleated Red Blood Cells and Anucleate Erythrocytes Using a Microfluidic Device," Journal of Chromatography A, 1217[11] (2010) 1862-1866

  78. J. Xie, Y. Zheng and J. Y. Ying, "Highly Selective and Ultrasensitive Detection of Hg2+ Based on Fluorescence Quenching of Au Nanoclusters by Hg2+-Au+ Interactions," Chemical Communications, 46[6] (2009) 961-963

  79. C. Iliescu, D. P. Poenar and S. T. Selvan, "Frequency Dependence on the Accuracy of Electrical Impedance Spectroscopy Measurements in Microfluidic Devices," Journal of Micromechanics and Microengineering, 20[2] (2009) 022001

  80. F. S. Iliescu, A. R. Sterian, E. Barbarini, M. Avram and C. Iliescu, "Continuous Separation of White Blood Cells from Blood in a Microfluidic Device UPB," UPB Scientific Bulletin Series A – Applied Mathematics and Physics, 71[4] (2009) 21-30

  81. Z. F. Chen and Y. Zu, "Electrogenerated Chemiluminescence of the Tris(2,2\'-bipyridine)ruthenium(II)/Tertiary Amine Systems: Effects of Electrode Surface Hydrophobicity on the Low-Oxidation-Potential Emission," Journal of Physical Chemistry C, 113[52] (2009) 21877-21882

  82. B. T. Chen, J. S. Wei and C. Iliescu, "Sonophoretic Enhanced Microneedle Array - Improving the Efficiency of Transdermal Drug Delivery," Sensors and Actuators B: Chemical, 145[1] (2009) 54-60

  83. X. J. Chen, H. Xie, Z. Y. Seow and Z. Q. Gao, "An Ultrasensitive DNA Biosensor Based on Enzyme-Catalyzed Deposition of Cupric Hexacyanoferrate Nanoparticles," Biosensors and Bioelectronics, 25[6] (2010) 1420-1426

  84. F. E. H. Tay, L. Yu and C. Iliescu, "Particle Manipulation by Miniaturized Dielectrophoretic Devices," Defence Science Journal, 59[6] (2009) 595-604

  85. C. Iliescu, G. Tresset and G. L. Xu, "Dielectrophoretic Field-Flow Method for Separating Particle Populations in a Chip with Asymmetric Electrodes," Biomicrofluidics, 3[4] (2009) 044104

  86. Y. B. Zu and Z. Q. Gao, "Facile and Controllable Loading of Single-Stranded DNA on Gold Nanoparticles," Analytical Chemistry, 81[20] (2009) 8523-8528

  87. H. Xie, S. C. Luo and H. H. Yu, "Electric-Field-Assisted Growth of Functionalized Poly(3,4-ethylenedioxythiophene) Nanowires for Label-Free Protein Detection," Small, 5[22] (2009) 2611-2617

  88. J. Wei, K. J. Leck, P. Gaughwin, M. Avram and C. Iliescu, "Low Stress Nanoporous SiNx Membrane for Cell Culture," Journal of Computational Materials Science and Surface Engineering, 2[3-4] (2009) 268-281

  89. B. P. Ting, J. Zhang, M. Khan, Y. Y. Yang, and J. Y. Ying, "The Solid-State Ag/AgCl Process as a Highly Sensitive Detection Mechanism for an Electrochemical Immunosensor," Chemical Communications, 41 (2009) 6231-6233

  90. S. Roy, X. J. Chen, M. H. Li, Y. F. Peng, F. Anariba and Z. Q. Gao, "Mass-Produced Nanogap Sensor Arrays for Ultrasensitive Detection of DNA," Journal of American Chemical Society, 131[34] (2009) 12211-12217

  91. B. P. Ting, J. Zhang, Z. Gao, and J. Y. Ying, "A DNA Biosensor Based on the Detection of Doxorubicin-Conjugated Ag Nanoparticle Labels Using Solid-State Voltammetry," Biosensors and Bioelectronics, 25 (2009) 282-287

  92. S. Roy and Z. Q. Gao, "Nanostructure-Based Electrical Biosensors," Nano Today, 4[4] (2009) 318-334

  93. F. Anariba, "Fabrication and Electrical Characterization of Molecular Junctions; A Robust Platform for Electron Transport Studies in Molecular Electronics," Verlag Dr. Mueller, (2009) DOI: -

  94. S. C. Luo, H. Xie, N. Y. Chen and H. H. Yu, "Trinity DNA Detection Platform by Ultrasmooth and Functionalized PEDOT Biointerfaces," ACS Applied Materials and Interfaces, 1[7] (2009) 1414-1419

  95. M. Bode, S. Khor, H. Ye, M.-H. Li and J. Y. Ying, "TmPrime: Fast, Flexible Oligonucleotide Design Software for Gene Synthesis," Nucleic Acids Research, 37 (2009) W214-W221

  96. Y. Zu, "Molecular and Nanoparticle Postcolumn Reagents for Assay of Low-Molecular-Mass Biothiols Using High-Performance Liquid Chromatograph," Journal of Chromatography B, 877[28] (2009) 3358-3365

  97. Y.-C. Toh, T. C. Lim, D. Tai, G. Xiao, D. van Noort and H. Yu, "A Microfluidic 3D Hepatocyte Chip for Drug Toxicity Testing," Lab on a Chip, 9[14] (2009) 2026-2035

  98. S.-C. Luo, J. Jiang, S. Liour, S. Gao, J. Y. Ying and H.-h. Yu, "Magnetic PEDOT Hollow Capsules with Single Holes," Chemical Communications, 19 (2009) 2664-2666

  99. J. Zhang, B. P. Ting, N. R. Jana, Z. Q. Gao and J. Y. Ying, "Ultrasensitive Electrochemical DNA Biosensors Based on the Detection of a Highly Characteristic Solid-State Process," Small, 5[12] (2009) 1414-1417

  100. H. Ye, M. C. Huang, M.-H. Li and J. Y. Ying, "Experimental Analysis of Gene Assembly with TopDown One-Step Real-Time Gene Synthesis," Nucleic Acids Research, 37[7] (2009) e51

  101. J. Xie, Y. Zheng and J. Y. Ying, "Protein-Directed Synthesis of Highly Fluorescent Gold Nanoclusters," Journal of the American Chemical Society, 131[3] (2009) 888-889

  102. C. Fang, Y. Fan, J. Kong, Z. Q. Gao and N. Balasubramanian, "Electrical Detection of Oligonucleotide Using an Aggregate of Gold Nanoparticles as a Conductive Tag," Analytical Chemistry, 80[24] (2008) 9387-9394

  103. C. Iliescu, G. L. Xu, E. Barbarini, M. Avram and A. Avram, "Microfluidic Device for Continuous Magnetophoretic Separation of White Blood Cells," Microsystem Technologies, 15[8] (2008) 1157-1162

  104. S.-C. Luo, H.-h. Yu, A. C. A. Wan, Y. Han and J. Y. Ying, "A General Synthesis for PEDOT-Coated Nonconductive Materials and PEDOT Hollow Particles by Aqueous Chemical Polymerization," Small, 4[11] (2008) 2051-2058

  105. M. C. Huang, H. Ye, Y. K. Kuan, M.-H. Li and J. Y. Ying, "Integrated Two-Step Gene Synthesis in a Microfluidic Device," Lab on a Chip, 9[2] (2008) 276-285

  106. H. H. Yu, M. K. F. Wong, E. Mohamed Ali and J. Y. Ying, "Photostable and Luminescent ZnO Films: Synthesis and Application as Fluorescence Resonance Energy Transfer Donors," Chemical Communications, 40 (2008) 4912-4914

  107. C. Fang, Y. Fan, J. Kong, Z. Q. Gao and N. Balasubramanian, "Preparation of Nanochain and Nanosphere by Self-Assembly of Gold Nanoparticles," Applied Physics Letters, 92[26] (2008) 263108

  108. A. Gole, N. R. Jana, S. T. Selvan and J. Y. Ying, "Langmuir-Blodgett Thin Films of Quantum Dots: Synthesis, Surface Modification and Fluorescence Resonance Energy Transfer (FRET) Studies," Langmuir, 24[15] (2008) 8181-8186

  109. S.C. Luo, E. Mohamed Ali, N. C. Tansil, H.-h. Yu, S. Gao, E. A. B. Kantchev and J. Y. Ying, "PEDOT Nanobiointerfaces: Thin, Ultrasmooth and Functionalized Poly(3,4-Ethylenedioxythiophene) Films with In Vitro and In Vivo Biocompatibility," Langmuir, 24[15] (2008) 8071-8077

  110. M. Avram, A. M. Avram, A Bragaru, A. Ghiu and C. Iliescu,, "Plasma Surface Modification for Selective Hydrophobic Control," Romanian Journal of Information Science and Technology, 11[4] (2008) 409-422

  111. C. Iliescu, J. Wei, B. Chen and P. L. Ong, "Silicon Nitride Membrane for Cell Culturing," Romanian Journal of Information Science and Technology, 11[2] (2008) 167-176

  112. Y. Shi, S.-C. Luo, W. Fang, K. Zhang, E. Mohamed Ali, F. Y. C. Boey, J. Y. Ying, J. Wang, H.-h. Yu and L. Li, "Work Function Engineering of Electrodes via Electropolymerization of Ethylenedioxythiophenes and Its Derivatives," Organic Electronics, 9[5] (2008) 859-863

  113. A. P. O'Mullane, J. Zhang, A. Brajter-Toth and A. M. Bond, "Higher Harmonic Large-Amplitude Fourier Transformed Alternating Current Voltammetry: Analytical Attributes Derived from Studies of the Oxidation of Ferrocenemethanol and Uric Acid at a Glassy Carbon Electrode," Analytical Chemistry, 80[12] (2008) 4614-4626

  114. J. Pipper, Y. Zhang, P. Neuzil and T. M. Hsieh, "Clockwork PCR Including Sample Preparation," Angewandte Chemie International Edition, 47[21] (2008) 3900-3904

  115. K. M. Schumacher, S. C. Phua, A. Schumacher and J. Y. Ying, "Controlled Formation of Biological Tubule Systems in Extracellular Matrix Gels In Vitro," Kidney International, 73[10] (2008) 1187-1192

  116. H. Taylor, D. Boning, C. Iliescu and B. Chen, "Computationally Efficient Modelling of Pattern Dependencies in the Micro-Embossing of Thermoplastic Polymers," Microelectronic Engineering, 85[5-6] (2008) 1453-1456

  117. S. F. Zhang, H. L. Leo, S. H. Kan and H. Yu, "Synthetic Collagen-free Sandwich Culture of Primary Hepatocytes based on Micro-fabrication Technology," Proceedings of the International Conference on Advances in Bioresorbable Biomaterials for Tissue Engineering, (2008) DOI: -

  118. Y. M. Khong, J. Zhang, S. B. Zhou, C. Cheung, K. Doberstein, V. Samper and H. Yu, "Novel Intra-Tissue Perfusion System for Culturing Thick Liver Tissue," Tissue Engineering, 13[9] (2007) 2345-2356

  119. Y. Han, L. Zhao and J. Y. Ying, "Entropy-Driven Helical Mesostructure Formation with Achiral Cationic Surfactant Templates," Advanced Materials, 19[18] (2007) 2454-2459

  120. Y. Zhang, K. C. Ngeow and J. Y. Ying, "The First N-Heterocyclic Carbene (NHC)-based Nickel Catalyst for C-S Coupling," Organic Letters, 9[18] (2007) 3495-3498

  121. J. Wei, P. L. Ong, F. E. H. Tay and C. Iliescu, "A New Fabrication Method of Low Stress PECVD SiNx Layers for Biomedical Application," Thin Solid Films, 516[16] (2008) 5181-5188

  122. C. Iliescu, B. T. Chen, J. Wei and A. J. Pang, "Characterization of Silicon Carbide Films Deposited by Plasma Enhanced Chemical Vapor Deposition," Thin Solid Films, 516[16] (2008) 5189-5193

  123. L. Kim, Y. C. Toh, J. Voldman and H. Yu, "A Practical Guide to Microfluidic Perfusion Culture of Adherent Mammalian Cells," Lab on a Chip, 7[6] (2007) 681-694

  124. G. Tresset and C. Iliescu, "Electrical Control of Loaded Biomimetic Femtoliter Vesicles in Microfluidic System," Applied Physics Letters, 90[17] (2007) 173901-1-173901-3

  125. K. Schumacher, Y. M. Khong, S. Chang, J. Ni, W. X. Sun and H. Yu, "Perfusion Culture Improves the Maintenance of Cultured Liver Tissue Slices," Tissue Engineering, 13[1] (2007) 197-205

  126. Y. C. Toh, C. Zhang, J. Zhang, Y. M. Khong, S. Chang, V. D. Samper, D. V. Noort, D. W. Hutmacher and H. Yu, "A Novel 3D Mammalian Cell Perfusion-Culture System in Microfluidic Channels," Lab on a Chip, 7[3] (2007) 302-309

  127. S. S. S. Ng, R. B. Han, J. Ni, S. Chang, W. Hunziker, A. B. Goryachev, S. H. Ong and H. Yu, "Improved Hepatocyte Excretory Function by Immediate Presentation of Polarity Cues," Tissue Engineering, 12[8] (2006) 2181-2191

  128. Y. N. Du, S. M. Chia and H. Yu, "3D Hepatocyte Monolayer on Hybrid RGD/Galactose Substratum," Biomaterials, 27[33] (2006) 5669-5680

  129. Y. C. Toh, S. S. S. Ng, Y. M. Khong, X. Zhang, Y. J. Zhu, P. C. Lin, C. M. Ten, W. X. Sun and H. Yu, "Cellular Responses to a Nanofibrous Environment," Nano Today, 1[3] (2006) 34-43

  130. H. L. Foo, A. Taniguchi, H. Yu, T. Okano and S. H. Teoh, "Catalytic Surface Modification of Roll-milled poly(ε-caprolactone) Biaxially Stretched to Ultra-thin Dimension," Materials Science and Engineering: C, 27[2] (2006) 299-303

  131. B. Annaz, K. A. Hing, M. V. Kayser, T. Buckland, and L. Di Silvio, "An Ultra-Structural Study of Cellular Response to Variation in Porosity in Phase-Pure Hydroxyapatite," Journal of Microscopy, 216[2] (2004) 97-109

  132. P. L. Mao, T. F. Liu, K. Kueh and P. Wu, "Predicting the Efficiency of UAG Translational Stop Signal Through Studies of Physicochemical Properties of its Composite Mono- and Di-Nucleotides," Computational Biology and Chemistry, 28[4] (2004) 245-256

  133. T. S. Pek, M. Spector, I. V. Yannas, and L. J. Gibson, "Degradation of a Collagen-Chondroitin-6-Sulfate Matrix by Collagenase and by Chondroitinase," Biomaterials, 25[3] (2003) 473-482

Research Staff

YING Jackie Yi-Ru, Executive Director

HIRAO Ichiro, Team Leader and Principal Research Scientist

TAN Min-Han, Team Leader and Principal Research Scientist

ZENG Huaqiang, Team Leader and Principal Research Scientist


15 Nov
Symposium on Science: Frontiers in Biology

6 Dec - 10 Dec
5th Nano Today Conference

Event Calendar   

One-North Run 2017


NEW! Genetic Alphabet Expansion Biotechnology by Creating Unnatural Base Pairs
Current Opinion in Biotechnology, 51 (2018) 8-15.
(IF: 9.294) article

NEW! Remanence Plots as a Probe of Spin Disorder in Magnetic Nanoparticles
Chemistry of Materials, (2017)
DOI: 10.1021/acs.chemmater.7b02522.
(IF: 9.466) article

NEW! Hyaluronic Acid-Green Tea Catechin Micellar Nanocomplex: Fail-Safe Cisplatin Nanomedicine for the Treatment of Ovarian Cancer Without Off-Target Toxicity
Biomaterials, (2017)
DOI: 10.1016/j.biomaterials.2017.09.027.
(IF: 8.402) article



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